An amino acid, alanine, may produce a short-term lowering of glucose levels by altering energy metabolism in the cell, scientists at Joslin Diabetes Center have found. Type 2 diabetes is driven by many metabolic pathways, with some pathways driven by amino acids, the molecular building blocks for proteins. The findings, published in Molecular Metabolism, show that it may be possible to use specific nutrients to change metabolism in a cell.
“Our study shows that it's possible we can use specific nutrients, in this case, amino acids, to change metabolism in a cell, and these changes in metabolism can change how cells take up and release glucose in a beneficial way,” says Mary-Elizabeth Patti, MD, an investigator in Joslin's Section on Integrative Physiology and Metabolism.
Performed in cells and mice, the research began with an attempt to see what nutrients might activate a key protein called AMP kinase (AMPK).
“AMPK is an enzyme in cells throughout the body that is activated when nutrient supplies are low, or in response to exercise,” says Patti, who is also an associate professor of medicine at Harvard Medical School.
“AMPK then causes a lot of beneficial changes in the cell, turning on genes that serve to increase energy production. AMPK is a good thing, and it also can be activated by a variety of treatments for Type 2 diabetes, such as metformin.”
The researchers then wanted to investigate whether an amino acid could “switch on” this beneficial enzyme.
The investigators began their study by testing many amino acids in rat liver cells, and found that alanine was the one amino acid that was consistently able to activate AMPK. The researchers then confirmed that AMPK was producing some of its usual metabolic effects after alanine activation. Additionally, the activation could be seen in human and mouse liver cells as well as rat liver cells and was present with either high or low levels of glucose in the cells.
Moreover, mice fed alanine showed a rise in levels of AMPK. If mice ate alanine before they received a dose of glucose, their resulting blood glucose levels were significantly lower. And while glucose metabolism often behaves quite differently in lean mice than in obese mice, this mechanism was seen in both groups of mice.
The Joslin team also found that the glucose-lowering didn't seem to be driven by increases in insulin secretion or decreases in the secretion of glucagon, a hormone that increases glucose. Instead, AMPK was boosting glucose uptake in the liver and decreasing glucose release. Further experiments in cells demonstrated that the activated enzyme was altering the Krebs cycle, a central component of cell metabolism.
“All these data together suggest that amino acids, and specifically alanine, may be a unique potential way to modify glucose metabolism,” Patti notes. “If it eventually turns out that you can do that by taking an oral drug as a pre-treatment before a meal, that would be of interest. However, this is early-stage research, and we need to test the concept both in mice and ultimately in humans.”